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Literature summary extracted from

  • Sim, R.B.; Porter, R.R.; Reid, K.B.M.; Gigli, I.
    The structure and enzymic activities of the C1r and C1s subcomponents of C1, the first component of human serum complement (1977), Biochem. J., 163, 219-227.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.42 0.07
-
 t-butyloxycarbonyl-Tyr p-nitrophenyl ester
-
 Homo sapiens
3.4.21.42 0.8
-
 N-benzyloxycarbonyl-Lys p-nitrophenyl ester
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.41 16600
-
 gel filtration Homo sapiens
3.4.21.41 83000
-
 2 * 83000, SDS-PAGE of reduced protein, gel filtration in 6 M guanidinium hydrochloride Homo sapiens
3.4.21.42 81000
-
 gel filtration Homo sapiens
3.4.21.42 92000
-
 nondenaturing PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.41 Homo sapiens
-
-
-
3.4.21.42 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.41 side-chain modification the A-chain contains 7.4% carbohydrate and the B-chain contains 12.4% carbohydrate Homo sapiens
3.4.21.42 side-chain modification 7.1% carbohydrate per weight Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.41 serum
-
 Homo sapiens
-
3.4.21.42 serum
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
3.4.21.41 2°C, pH 5.3-7.3, in absence of Ca2+, 2 weeks, stable Homo sapiens
3.4.21.42 2°C, pH 5.3-7.3, stable over weeks Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.41 complement component C1s + H2O C1rbar does not hydrolyze any amino acid ester tested nor any protein substrate except subcomponent C1s Homo sapiens complement component C1sbar
-
 ?
3.4.21.42 N-benzyloxycarbonyl-Lys-p-nitrophenyl ester + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.42 tert-butyloxycarbonyl-Lys p-nitrophenyl ester + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.42 tert-butyloxycarbonyl-Phe p-nitrophenyl ester + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.42 tert-butyloxycarbonyl-Tyr p-nitrophenyl ester + H2O
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.41 dimer 2 * 83000, SDS-PAGE of reduced protein, gel filtration in 6 M guanidinium hydrochloride Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.42 0.73
-
 tert-butyloxycarbonyl-Tyr p-nitrophenyl ester
-
 Homo sapiens
3.4.21.42 20.8
-
 N-benzyloxycarbonyl-Lys p-nitrophenyl ester
-
 Homo sapiens